Abstract: Hydrological regime plays a crucial role in river ecologic systems. Analysis on the effects of dams on the regime of a river using the range of variability approach (RVA) can provide key information for protection of its ecological integrity. In this study, this approach was applied to hydrological regime changes at the Xiangyang and Yicheng stations on the midstream Han River in the period of 1952-2013. We selected the year 1974 as the change point and divide for analysis of hydrological deviation rate and hydrological change, and analyzed the midstream hydrological changes using five sets of hydrological indexes: runoff level, frequency, duration, time of occurrence, and distribution of change rate. The results show that under the control of Danjiangkou dam that started in 1974, the water levels at these two stations were generally lower, and the change in the water level was most significant at Xiangyang while mild at Yicheng. This study would be useful for evaluation of the downstream of Han River and water resources management.